http://hdl.handle.net/123456789/278 2026-05-15T08:32:26Z 2026-05-15T08:32:26Z Mosiichuk, Nadiia Мосійчук, Надія Михайлівна http://hdl.handle.net/123456789/2861 2023-02-28T10:27:40Z 2015-01-01T00:00:00Z

Title: Effects Of Sodium Nitroprusside On Salt Stress Tolerance Of Tocopherol-Deficient Arabidopsis Thaliana Plants Authors: Mosiichuk, Nadiia; Мосійчук, Надія Михайлівна Abstract: In the present study, effects of exogenous sodium nitroprusside (SNP), a nitric oxide (•NO) donor, on lipid peroxidation and antioxidant enzyme activities in wild type and tocopherol-deficient lines vte1 and vte4 of Arabidopsis thaliana subjected to 200 mM NaCl were studied. In wild type plants, pretreatment with SNP did not change level of thiobarbituric acid reactive substances (TBARS), but decreased the activities of dehydroascorbate reductase and guaiacol peroxidase under salt stress. In mutant line vte1, which lacks all forms of tocopherols, pretreatment with SNP reduced TBARS level and increases the activities of glutathione reductase and guaiacol peroxidase under salt stress. Ascorbate peroxidase activity decreased under salt stress conditions in both mutant lines, pretreated with SNP. It can be concluded, that pretreatment with SNP could attenuate salt-induced injuries in A. thaliana plants via up-regulation of activity of antioxidant enzymes and attenuate lipid peroxidation.

2015-01-01T00:00:00Z Shmihel, Halyna http://hdl.handle.net/123456789/2860 2020-03-30T13:50:32Z 2015-01-01T00:00:00Z

Title: Alpha-Ketoglutarate Partially Protects Fruit Fly Drosophila Melanogaster From Ethanol Toxicity Authors: Shmihel, Halyna Abstract: Alpha-ketoglutarate (AKG) is an important intermediate in Krebs cycle and in metabolism of amino acids. Recently, it was proposed to apply as a dietary supplement to improve overall functional state of living organisms. In particular, AKG was supposed to use under exposure of animals and cell cultures to many toxic agents. In this context, this study aimed to elucidate the ability of dietary AKG to reduce toxic effects of ethanol on development of fruit fly Drosophila melanogaster, which is a popular model subject to research many aspects of biology of higher eukaryotes. For this aim, the effect of sodium salt of AKG on pupation speed of D. melanogaster w1118 on the medium supplemented with different concentrations of ethanol was studied. Ethanol at a low concentration (2%) did not affect the rate of larval pupation, whereas at higher concentrations it significantly delayed fly pupation and showed developmental toxicity reducing a number of total pupae formed. The most toxic developmental effects of ethanol were observed at its highest concentration (15%). The potential mechanisms of protective effects of AKG are discussed.

2015-01-01T00:00:00Z Vasylkovska, Ruslana Burdyliuk, Nadia Semchyshyn, Halyna Семчишин, Галина Миколаївна http://hdl.handle.net/123456789/2859 2025-07-02T07:14:29Z 2015-01-01T00:00:00Z

Title: Involvement Of Catalase In Saccharomyces Cerevisiae Hormetic Response To Hydrogen Peroxide Authors: Vasylkovska, Ruslana; Burdyliuk, Nadia; Semchyshyn, Halyna; Семчишин, Галина Миколаївна Abstract: In this study, we investigated the relationship between catalase activity and H2O2-induced hormetic response in budding yeast S. cerevisiae. In general, our data suggest that: (i) hydrogen peroxide induces hormesis in a concentration- and time-dependent manner; and (ii) the effect of hydrogen peroxide on yeast colony growth positively correlates with the activity of catalase that suggests the enzyme involvement in overall H2O2-induced stress response and hormetic response in yeast.

2015-01-01T00:00:00Z Abrat, Oleksandra http://hdl.handle.net/123456789/2858 2020-03-30T13:30:56Z 2015-01-01T00:00:00Z

Title: Influence Of Amylose Starch On Development And Lifespan Of Fruit Fly Drosophila Melanogaster Authors: Abrat, Oleksandra Abstract: Last years, the concept of resistant starch (RS) has evoked a new interest in researchers in the context of bioavailability of starch and its use as a source of dietary fiber. Based on clinical and animal research, RS has been proposed to be the most potentially beneficial starch fraction for human health. In this study, the effects of amylose starch as a fraction of RS on development and lifespan of fruit fly Drosophila melanogaster were investigated. In both Canton S and w1118 strains, the diet with 20% amylose RS delayed fly development, increased triacylglyceride level in the body of adult insects and reduced their lifespan compared to the diet with 4% amylose starch. Thus, our data clearly demonstrate that amylose starch at high concentrations may negatively affect fruit fly.

2015-01-01T00:00:00Z